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The complete nutrient medium was comprised of phenol red-containing Dulbecco’s Modified Eagle’s medium (DMEM, Lonza) with 4.5 g/L glucose, L-glutamine and supplemented with fetal bovine serum (FBS, Sigma-Aldrich) to a 10% final concentration and penicillin/streptomycin mixture to final concentrations of 100 U/mL for each of them

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Calculation of ICC and Heritability For twin studies the one-way model of intra-class correlation (ICC) is used to determine the similarity of a measure in a twin pair, ICC = (MS between − MS within ) / (MS between + MS within ), where MS between is the estimate of the mean-square variance between all twin-pairs and MS within is the estimate of the mean-square variance within the sets of pairs in that group [23]